Deposition of living biological tissue. https://reprap.org/forum/list.php?194 Tue, 14 Jul 2026 00:22:56 -0400 Phorum 5.2.23 https://reprap.org/forum/read.php?194,856949,856949#msg-856949 https://reprap.org/forum/read.php?194,856949,856949#msg-856949
Many thanks for your help

Jim Gillies]]> jimgillies Tissue Engineering Tue, 23 Jul 2019 07:33:03 -0400 https://reprap.org/forum/read.php?194,840362,840362#msg-840362 https://reprap.org/forum/read.php?194,840362,840362#msg-840362
Quick questions for all you bioprinting experts/ tissue engineers: 1. In bioprinting does the current technology allow for the printing of non-human tissue such as animal bone? When printing scaffolds for post-seeding, is there any way the scaffold can be removed/washed out after full tissue generation or will the scaffold material be imbedded into the tissue for good; and 3. Can cells scrapped off the outer surface of tissue (e.g.: bone, skin), be used to successfully seed a scaffold?]]> Eardnua1 Tissue Engineering Mon, 03 Dec 2018 22:54:27 -0500 https://reprap.org/forum/read.php?194,780082,780082#msg-780082 https://reprap.org/forum/read.php?194,780082,780082#msg-780082
I have been designing a 3D printer that's on the same infrastructure as Prusa i3 for bioprinting. However, I am not sure how to use my new extruder, which is a pressure-based solenoid valve.

Essentially, the valve is powered by a Spike and Hold, not a PID like Prusa i3 is. This spike and hold requires 24 V in the first millisecond and then 5 V for the hold voltage. As you already know, Arduino cannot exceed 24 V or else it will burn out the circuit. In addition, the Marlin software that powers this model is for PID.

My question is this: how can I redesign the printer for the solenoid signal without damaging the Arduino?]]> JoshuahMG Tissue Engineering Wed, 10 Jan 2018 23:49:59 -0500 https://reprap.org/forum/read.php?194,756562,756562#msg-756562 https://reprap.org/forum/read.php?194,756562,756562#msg-756562
Is there a way I can customize the infill % to define the separation between the lines to form a grid with the pore sizes of the bone?
I have been looking some approaches using matlab to generate the mathematical model of the scaffold and generating a STL file
Another approach is using topology optimisation and sphere packing algorithms,
Before I get my hands dirty, I wanted to ask if there is a easier method for generating a scaffold printings settings.


I'm really new into this field, so any information or documentation is welcome so I can expand this project.

Thanks,

Alfonso]]> peb90 Tissue Engineering Fri, 17 Mar 2017 12:48:04 -0400 https://reprap.org/forum/read.php?194,665571,665571#msg-665571 https://reprap.org/forum/read.php?194,665571,665571#msg-665571
I am looking for an adhesive to fix tissue parts firmly onto a plastic substrate. I need to fix tissue parts in place during mechanical test.
Could you please introduce me a good adhesive for this purpose?

Thanks in advance. =)]]> Moones Tissue Engineering Thu, 19 May 2016 06:52:17 -0400 https://reprap.org/forum/read.php?194,556889,556889#msg-556889 https://reprap.org/forum/read.php?194,556889,556889#msg-556889
I am currently working on a project that aims to develop bioactive glass-based porous coatings/surfaces for bone tissue engineering. The thickness of the coating is in the range of 100-500 nanometers. The major problem I encountered is that my coatings disappear few hours after putting them in deionized water. However when making the same nano-featured coatings using silica only the coating was stable for almost 2 weeks without remarkable damage!

For experts in bioactive glass for bone tissue Engineering, would you help me understand why the presence of CaO renders the bioactive glass Network unstable in water ? Thank you very much in advance!

The bioactive glass I am using is the binary composition of SiO2 and CaO (70% of SiO2 and 30% of CaO).


Best,
Houda]]> Hoditta Tissue Engineering Tue, 26 Jan 2016 14:21:34 -0500 https://reprap.org/forum/read.php?194,556118,556118#msg-556118 https://reprap.org/forum/read.php?194,556118,556118#msg-556118 www.appropedia.org]) but I'm not sure how to to do the circuitry or what to change in the arduino code. Im using Arduino Megan and RAMPS 1.4 with drivers.

-How would i connect the syringe pump motor to the RAMPS?
-im using the Marlin Firmware-how can i adjust the coding for no thermal components but just the syringe pump?
-is there any open source coding out there for a similar design?

Would really appreciate some help
thanks]]> gkoshy Tissue Engineering Tue, 22 Dec 2015 16:39:06 -0500 https://reprap.org/forum/read.php?194,429491,429491#msg-429491 https://reprap.org/forum/read.php?194,429491,429491#msg-429491 Thank You.]]> lezlycross Tissue Engineering Mon, 01 Dec 2014 07:18:58 -0500 https://reprap.org/forum/read.php?194,429478,429478#msg-429478 https://reprap.org/forum/read.php?194,429478,429478#msg-429478 lezlycross Tissue Engineering Tue, 07 Apr 2015 12:58:18 -0400 https://reprap.org/forum/read.php?194,405908,405908#msg-405908 https://reprap.org/forum/read.php?194,405908,405908#msg-405908
PCL solution was formed by dissolving PCL pellets in chloroform to the concentration of 15%. Sodium bicarbonate salt (acting as porogen leaching and effervescence salt) was then added in a variety of ratios to the PCL solutions. Ethanol was used as a non-solvent to precipitate out the PCL to form a gel paste, which was then placed into a mold. The next step would be to immerse the mold in citric acid to react away the salt and create effervescence which is supposed to help expand the pore size within the PCL scaffold. Herein lies the problem:

1) Although the sodium bicarbonate salt was supposed to be mixed homogeneously within the PCL solution, it was observed to all settle down to the bottom of the mold/petri dish after awhile. So essentially there's a bottom layer of settled salt and a top layer with no salt at all. (Any recommendations on how to improve on this step?)

2) When the carbon dioxide was formed, it "forced" its way out of the mold through the surface, creating large holes at the sides and on the top layer. This action essentially broke the integrity of the PCL mold/scaffold.

3) After effervescence was completed, what was left was only a thin layer of PCL (I have no idea why this is so)

Because of all these factors, I am unable to create the desired scaffold. Could anyone kindly provide me with some directions / point out any possible mistakes in my steps? Greatly appreciated!]]> seet Tissue Engineering Wed, 05 Nov 2014 14:23:54 -0500 https://reprap.org/forum/read.php?194,292182,292182#msg-292182 https://reprap.org/forum/read.php?194,292182,292182#msg-292182
There is a link of youtube video of showing how to use RepRap printer to print vascular network out of sugar ( [www.youtube.com] ). In my FYP, I also have to achieve the purpose which is similar to this thing.
In this papar, the sugar is carbohydrate glass which should be kept at 110C to be kept in liquid state for printing. This means the syringe must be heated up to that temperature, and the sugar is pressed out of syringe under the pnuematic system such as pnuematic solenoid valve.Check this: [www.flickr.com]

Therefore, I would also like to know what and how can I do to replace the hot end assembly of RepRap huxley printer by the new designed syringe with pnuematic solenoid? And also, what should I and where can I modify in gcode to suit this work as I am new in learning these things?

Thanks!]]> Oscar Tissue Engineering Thu, 16 Feb 2017 05:39:47 -0500 https://reprap.org/forum/read.php?194,226741,226741#msg-226741 https://reprap.org/forum/read.php?194,226741,226741#msg-226741
[www.nature.com]

These guys used detergents to strip kidney tissue to a structural scaffold then reculture stem cells onto them. My thoughts are to evaluate if it is possible to build that initial scaffold from a 3D printer. Has anyone taken a look at building scaffolds, biogels, sugar lattices, or bioreactors, in concert with using a reprap?

-Soon to be american physician with a background in biomedical engineering that can't sit still for 5 minutes]]> An0Ma|Y Tissue Engineering Thu, 09 Apr 2015 13:02:05 -0400 https://reprap.org/forum/read.php?194,211847,211847#msg-211847 https://reprap.org/forum/read.php?194,211847,211847#msg-211847
I don't know a lick about this, but I hope some one out there has an idea on how to go about it. The applications for printing plants could be ground breaking. World hunger, global warming, teraforming mars, who knows?

Can it be done? How do we make it happen?]]> Anonymous User Tissue Engineering Sat, 05 Oct 2013 10:08:22 -0400 https://reprap.org/forum/read.php?194,207482,207482#msg-207482 https://reprap.org/forum/read.php?194,207482,207482#msg-207482
Ideally I think I am looking for something that would survive an autoclave, alcohol and the odd bit of bleach. Any ideas? - Possibly Polycarbonate.

The follow up question is, what would you need to upgrade over a standard reprap kit (thinking reprap pro Huxley here).

---

So far the ideal plastic appears to be Polyphenylsulfone (PPSF/PPSU) but I may have swallowed someones marketing hype, but it seems to run at the $400 per 1litre, and only available in canisters for a specific printer....]]> squeeks Tissue Engineering Fri, 16 Aug 2013 07:59:28 -0400 https://reprap.org/forum/read.php?194,202283,202283#msg-202283 https://reprap.org/forum/read.php?194,202283,202283#msg-202283
reading the "print a plant" topic I remembered something from my urban art days, I don't see why this couldn't be used with 3d printing technology, how to make moss graffiti
or a youtube video

seems simple enough with the right extruder, printing on tiles then transferring them to the outdoors. I figure someone here could use this information better than I can. xD what do you guys think?]]> savvy Tissue Engineering Tue, 23 Apr 2013 16:44:07 -0400 https://reprap.org/forum/read.php?194,202073,202073#msg-202073 https://reprap.org/forum/read.php?194,202073,202073#msg-202073 www.3ders.org]]]> VDX Tissue Engineering Tue, 23 Apr 2013 03:12:47 -0400 https://reprap.org/forum/read.php?194,198904,198904#msg-198904 https://reprap.org/forum/read.php?194,198904,198904#msg-198904
Thanks :-)]]> Ati Tissue Engineering Tue, 07 Apr 2015 12:52:31 -0400 https://reprap.org/forum/read.php?194,197225,197225#msg-197225 https://reprap.org/forum/read.php?194,197225,197225#msg-197225
A 3D Printer Gave A Man His Face (And Life) Back]]> Mogal Tissue Engineering Thu, 18 Apr 2013 14:06:40 -0400 https://reprap.org/forum/read.php?194,190207,190207#msg-190207 https://reprap.org/forum/read.php?194,190207,190207#msg-190207 www.patexia.com]]]> maria07 Tissue Engineering Wed, 06 Mar 2013 11:55:34 -0500 https://reprap.org/forum/read.php?194,188943,188943#msg-188943 https://reprap.org/forum/read.php?194,188943,188943#msg-188943
If you’re familiar with the technology, or interested in developing your patent search skills, the winner is going to receive $3000 for submitting the best entry.

We’re also offering a $500 prize for referring the winner through our site, so feel free to invite your colleagues instead if you know the right person.

You can see more details and figure out if the study is a match for you here: [bit.ly]]]> maria07 Tissue Engineering Sat, 02 Mar 2013 11:57:54 -0500 https://reprap.org/forum/read.php?194,168988,168988#msg-168988 https://reprap.org/forum/read.php?194,168988,168988#msg-168988
First post in the forums...

I am starting my senior project in college and am researching best methods for tissue scaffolds and the printers to use for them. I am looking for a printer that is at the most 50 mm. I have built the Pro Huxley and Mendel thus far, do I need different parts to print on a smaller scale?

Just wondering if anyone has had success in doing so with a RepRap printer and which model...very vague apologies in advance.]]> burketommy Tissue Engineering Sat, 08 Nov 2014 03:56:14 -0500 https://reprap.org/forum/read.php?194,166193,166193#msg-166193 https://reprap.org/forum/read.php?194,166193,166193#msg-166193 I'm doing the senior project on Material Science and really don't know where to look for this piece of information. The material I'm working on is Polyglycolic acid. Does anyone have any ideas what additives should not be there for it to be considered as medical grade? References would also be helpful.
Thanks in advance.]]> laguz Tissue Engineering Thu, 22 Nov 2012 07:24:46 -0500 https://reprap.org/forum/read.php?194,151423,151423#msg-151423 https://reprap.org/forum/read.php?194,151423,151423#msg-151423
[on3dprinting.com]]]> richrap Tissue Engineering Thu, 30 Aug 2012 11:01:17 -0400 https://reprap.org/forum/read.php?194,145066,145066#msg-145066 https://reprap.org/forum/read.php?194,145066,145066#msg-145066
check this out - a modified reprap printing a 3d blood vessel scaffold! and this concept.

Does anyone know any more about how the print material is formulated and then delivered to the extrusion apparatus? Any speculation about the extrusion assembly?

See: (Bhatia and Chen 2012)

Bhatia, S. N. & C. S. Chen (2012) Rapid casting of patterned vascular networks for perfusable engineered three-dimensional tissues.

I have this paper and advise reading it - I think it is a really big deal for both 3d bioprinting and the reprap - pm me for more information.

Rgds,

Patrick]]> paddy10tellys Tissue Engineering Fri, 05 Oct 2012 07:38:13 -0400 https://reprap.org/forum/read.php?194,123922,123922#msg-123922 https://reprap.org/forum/read.php?194,123922,123922#msg-123922
I am looking to investigate cell seeding and want to initially test a range of bone scaffolds. The idea of using reprap for some of these is very exciting, but I understand that there are some limitations.

I would like to mimic the structure of trabecular bone as much as possible (structural properties less important), but I am given to understand from other posts and papers that it may be an issue to get the required trabecular strut thickness?

Also, it is possible to produce a reticulated structure, somewhat similar to an open cell foam? I am fairly flexible on the materials to be used, but am immersion testing

Thanks, any advice is welcomed]]> newmansown Tissue Engineering Tue, 13 Mar 2012 10:09:14 -0400 https://reprap.org/forum/read.php?194,110352,110352#msg-110352 https://reprap.org/forum/read.php?194,110352,110352#msg-110352 LXDickman Tissue Engineering Mon, 19 Dec 2011 11:37:26 -0500 https://reprap.org/forum/read.php?194,77320,77320#msg-77320 https://reprap.org/forum/read.php?194,77320,77320#msg-77320
From what I know right now, there are quite a few challenges to meeting this objective...

1. Make a RepRap that's accurate to 10 micrometers (.01mm?)
2. What kind of print head design would work best to print an animal cell? a plant cell?
3. How do we make the system stitch cells into tissues?

On the other side of the printing, we need to figure out scanning and structural systems.
4. What kind of scanning do we have available for living tissues?
5. What modeling systems and software do we have to simulate and analyze cell and organ structures and interactions?

These are a few questions that need to be answered, and as more research comes along, I'll post my progress.]]> azeem.hussein Tissue Engineering Mon, 21 Jan 2013 11:09:15 -0500 https://reprap.org/forum/read.php?194,75943,75943#msg-75943 https://reprap.org/forum/read.php?194,75943,75943#msg-75943
[www.physorg.com]]]> OliverJenkins Tissue Engineering Thu, 10 Mar 2011 16:41:42 -0500 https://reprap.org/forum/read.php?194,75225,75225#msg-75225 https://reprap.org/forum/read.php?194,75225,75225#msg-75225
I see a lot of discussion here about printing tissues organs etc....
Most discussed material is PCL (aka polycaprolactone, CAPA, polymporph). To my knowledge it is hard wax (at RT) like
material so I really doubt if anybody of forum participants have got a reel of pure PCL filament and printed. If I'm wrong please share your experience here.
Other popular choices in tissue engineering world are PLA (polylactic acid) and PGA (polyglicolic acid) if we are not discussing gel like exotics like alginate, hyaluronan, chitosan etc.
If you plan to make something biocompatible and usable in the medical or at least vetetinary practice, you must have clean
medical grade raw materials. The question is where to get these at affordable prices? In RapMan community it is common to use PLA from BFB, RepRapsource or Orbitech. These guys are only reselers or filament producers at most. Actual material they use, is produced by the worlds largest PLA manufacturer Nature Works LLC. NW does not disclose material purity or composition, just a technological info. Manufacturers price is 3 eur/kg in bulk quantities and 6 eur/kg with VAT at retail. Materials we put in our machines are PLA 4043D or 4032D, why they were choosen by filament producers remains unknown, althoug other PLA from Natureworks is pretty similar in characteristics. If you have some knowledge about that please do not hesitate to share here.
Turning back to organ printing matters it is hard to get medical grade PLA or PGA in quantities and form we need for printing. There are some suppliers of medical polymers like Purac.com , but prices reach the sky. Once you mention word medical - everything become 10x priced like it was gold plated, albeit was taken from the same garage same box like industrial instrument or material. I do not want to overgeneralize that statement, since many times medical grade materials have justified prices. But just want to learn those rare sources of materials where we can get clean material at reasonable prices. Any thoughts welcome.]]> Andrius Tissue Engineering Tue, 29 Oct 2013 15:53:44 -0400 https://reprap.org/forum/read.php?194,74522,74522#msg-74522 https://reprap.org/forum/read.php?194,74522,74522#msg-74522 bioskin bbc]]> richrap Tissue Engineering Mon, 21 Feb 2011 17:58:58 -0500