There are two ways.
For the calculator you linked:
R0 and T0 are a resistance/temperature pair taken from the data sheet. Normally the 25C values.
Beta is taken from the data sheet.
R1 = zero , I don't think any current reprap electronics have an R1.
R2 = the sense resistor from your electronics, which is 4700 ohms as standard.
But a more accurate way is to use the script from this article:
[
hydraraptor.blogspot.co.uk]
You need python installed on your computer, which you already do if you use skeinforge or printrun. The easy way to use it is to load it in IDLE and alter the parameters directly. This script takes three resistance/temperature pairs, as well as the value of the sense resistor on your electronics, which again is normally 4700 ohm.