Your steps per mm shouldn't be a guess, and 320 sounds like the microstepping isn't setup correctly. Your steps pe rmm should be calculated based off of the motor step angle, the microstepping, and the screw pitch
Unless you've got wierd motors, they're gonna be Nema 17, with 1.8 degree step angle
If you're using a RAMPS based board, make sure you've got all 3 jumpers placed under the Z Axis Stepper Driver, that'll be 1/16 microstepping
Last thing in question would be the size of the screw
Knowing that, use the
Prusa Calculator to determine your steps per mm
And put that in your configuration file
Also make sure the feed rate isn't too high,